Genbank

 

Genbank

Aim

To retrieve information for a given organism from Genbank

Description

GenBank is the NIH genetic sequence database, an annotated collection of all publicly available DNA sequences .It is a primary database of nucleotide. GenBank is part of the International Nucleotide Sequence Database Collaboration, which comprises the DNA Data Bank of Japan (DDBJ), the European Nucleotide Archive (ENA), and GenBank at NCBI. These three organizations exchange data on a daily basis. A GenBank release occurs every two months and is available from the ftp site. The release notes for the current version of GenBank provide detailed information about the release and notifications of upcoming changes to GenBank. Release notes for previous GenBank releases are also available. Here information starts with the line containing the word “LOCUS”.

Procedure

STEP 1: Open  https://www.ncbi.nlm.nih.gov/genbank/

STEP 2: Select Genbank from NCBI homepage.

STEP 3: Choose nucleotide from the dropdown list and type E.coli in the search box and click on the top

STEP 4: Note down the accession number, Locus, bp length, nucleotide type.

STEP 5: Save the page

STEP 6: Close the window

Result

Nucleotide sequence information retrived from Genbank.

 

LOCUS           :  LZ177092 

Basepair length : 17 bp
Nucleotide      :  DNA linear
Accession number: LZ177092
Defnition       : JP 2017136019-A/18: CARRIER FOR DETECTING PATHOGENIC ESCHERICHIA COLI BACTERIA, KIT FOR DETECTING PATHOGENIC ESCHERICHIA COLI BACTERIA AND METHOD FOR DETECTING PATHOGENIC ESCHERICHIA COLI BACTERIA.
 

 

 

 

Comments

Post a Comment

Popular posts from this blog

Cell membrane /Plasma membrane

Image
    Plasma membrane or cell membrane Plasma membrane or cell membrane is a dynamic , fluid structure and forms external boundary of the cell. It acts as a selective permeable membrane and regulates molecular traffic across the boundary. Plasma membrane behaves as a capacitor. In absence of integral membrane proteins, plasma membrane have similar electrical properties. The non conductive lipid bilayer of the membrane separates the conductive extracellular and intracellular electrolyte solutions. This results in the separation of charge across the lipid bilayer and this charge separation   brings about an electrical potential difference across the bilayer which is referred to as membrane potential. Different models are: Overton model The Overton model, (1899)  proposed by Charles Overton, suggests that the plasma membrane is primarily composed of lipids, and its permeability is largely determined by the lipid solubility of substances. This model is based...
Read more

Bovine Spongiform Encephalopathy (BSE)

Image
  Bovine Spongiform Encephalopathy (BSE) Spongiform encephalopathies (SEs) are degenerative disorders of the brain that occur in a number of species. They are recognized by the clinical appearance of the affected animal and the characteristic histological changes they produce in the brain.  One of the common Spongiform encephalopathy is Bovine Spongiform Encephalopathy (BSE),which mainly affect cattle. Bovine Spongiform Encephalopathy (BSE), widely referred to as “mad cow disease,” is a progressive and fatal neurologic disease of cattle. It is caused by  an unconventional transmissible agent, an abnormal prion protein . "Bovine" means that the disease affects cows, "spongiform" refers to the way the brain from a sick cow looks spongy under a microscope, and "encephalopathy" indicates that it is a disease of the brain. The infection spreads to cattle by the practice of feeding them abattoir waste and offal which may have contained scrapie infected meat. ...
Read more

Biological databases

 Biological databases A database is a computerized archive used to store and organize data in such a way that information can be retrieved easily via a variety of search criteria. Databases are composed of computer hardware and software for data management. The chief objective of the development of a database is to organize data in a set of structured records to enable easy retrieval of information. Each record, also called an entry, should contain a number of fields that hold the actual data items, for example, fields for names, phone numbers, addresses, dates. To retrieve a particular record from the database, a user can specify a particular piece of information, called value , to be found in a particular field and expect the computer to retrieve the whole data record. This process is called making a query. Biological databases are  libraries of biological sciences, collected from scientific experiments, published literature, high-throughput experiment technology, and compu...
Read more