PIR

 

PIR

Aim

To retrieve aminoacid sequence for heat shock protein HSP70 in tomato.

Description

The protein information resource(PIR),located at Georgetown University Medical Center (GUMC), is an integrated public bioinformatics resource to support genomic  and proteomic research and scientific studies.

PIR was established in 1984 by the National Biomedical Research Foundation(NBRF) as a resource to assist researchers and consumers in the identification and interpretation of protein sequence information. Prior to that ,the NBRF compiled the first comprehensive collection of macromolecular sequences n the Atlas of protein sequence and structure, published from 1964-1974,under the editorship of Margaret Dayhoff. Dr. Dayhoff and her research group pioneered in the development of computer methods for the comparison of protein sequences, for the detection of distantly related sequences and duplications within sequences and for the inference of evolutionary histories from alignments of protein sequence. In 2002, PIR along with its international partners ,EBI (European bioinformatics Institute) and SIB (Swiss Institute  of Bioinformatics  were awarded or grant from NIH to create Uniprot as a single worldwide database of protein sequence and function by unifying PIR-PSD, Swissport and TrEMBL databases.

Procedure

 

STEP 1: Access https://proteininformationresource.org/

STEP 2: Enter the keyword HSP70 tomato in the search box at the left corners of the page and search

STEP 3: Click on the required entry to retrieve related information.

STEP 4: Close the window

Result

The amino acid sequence and the related information for the heat shock protein in tomato was successfully retrieved.                

Comments

Post a Comment

Popular posts from this blog

Cell membrane /Plasma membrane

Image
    Plasma membrane or cell membrane Plasma membrane or cell membrane is a dynamic , fluid structure and forms external boundary of the cell. It acts as a selective permeable membrane and regulates molecular traffic across the boundary. Plasma membrane behaves as a capacitor. In absence of integral membrane proteins, plasma membrane have similar electrical properties. The non conductive lipid bilayer of the membrane separates the conductive extracellular and intracellular electrolyte solutions. This results in the separation of charge across the lipid bilayer and this charge separation   brings about an electrical potential difference across the bilayer which is referred to as membrane potential. Different models are: Overton model The Overton model, (1899)  proposed by Charles Overton, suggests that the plasma membrane is primarily composed of lipids, and its permeability is largely determined by the lipid solubility of substances. This model is based...
Read more

Bovine Spongiform Encephalopathy (BSE)

Image
  Bovine Spongiform Encephalopathy (BSE) Spongiform encephalopathies (SEs) are degenerative disorders of the brain that occur in a number of species. They are recognized by the clinical appearance of the affected animal and the characteristic histological changes they produce in the brain.  One of the common Spongiform encephalopathy is Bovine Spongiform Encephalopathy (BSE),which mainly affect cattle. Bovine Spongiform Encephalopathy (BSE), widely referred to as “mad cow disease,” is a progressive and fatal neurologic disease of cattle. It is caused by  an unconventional transmissible agent, an abnormal prion protein . "Bovine" means that the disease affects cows, "spongiform" refers to the way the brain from a sick cow looks spongy under a microscope, and "encephalopathy" indicates that it is a disease of the brain. The infection spreads to cattle by the practice of feeding them abattoir waste and offal which may have contained scrapie infected meat. ...
Read more

Biological databases

 Biological databases A database is a computerized archive used to store and organize data in such a way that information can be retrieved easily via a variety of search criteria. Databases are composed of computer hardware and software for data management. The chief objective of the development of a database is to organize data in a set of structured records to enable easy retrieval of information. Each record, also called an entry, should contain a number of fields that hold the actual data items, for example, fields for names, phone numbers, addresses, dates. To retrieve a particular record from the database, a user can specify a particular piece of information, called value , to be found in a particular field and expect the computer to retrieve the whole data record. This process is called making a query. Biological databases are  libraries of biological sciences, collected from scientific experiments, published literature, high-throughput experiment technology, and compu...
Read more